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1. Principle of two-dimensional liquid chromatography (2D-LC)
The principle behind 2D-LC is that fractions from a first dimension (denoted 1 D) are transferred to a second dimension (denoted 2 D) for a second separation. Ideally, the two separations are complementary, bringing different selectivities to the compounds to be separated.
1.1 2D-LC modes
There are two main modes in 2D-LC. The first is called heart-cut (LC-LC), while the second is called comprehensive (LC×LC). For ease of reference, the terms heart-cut and comprehensive are used throughout this article. Figure 1 illustrates these two...
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Principle of two-dimensional liquid chromatography (2D-LC)
When to choose LC×LC over LC?
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