3. Contributions to peak broadening
Capillary electrophoresis is an analytical technique renowned for giving high efficiency values, in excess of 10 4 , or even 10 5 . This stems from the flat velocity profile of electroosmotic flow, which minimizes the dispersion of a band of a given compound along the capillary as it moves compared to a parabolic hydrodynamic flow as observed in liquid chromatography. However, several phenomena can quickly contribute to a drop in efficiency, degrading the shape of peaks and the appearance of the electrophoregram. This section describes the main phenomena that can widen and distort peaks, and the options for avoiding them, based on the assumption that each compound is characterized by a Gaussian-shaped peak. In this case, the width of the peak is characterized by the standard deviation σ, knowing that...
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Contributions to peak broadening
Bibliography
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Norms and standards (non-exhaustive list)
- [IDF 206:2006] Milk powder – Determination of soybean and pea proteins by capillary electrophoresis in the presence of sodium dodecyl sulfate (SDS-CE) – Screening method - ISO 17129 - 2006
- Powdered milk-based infant formulas – Quantification of whey protein content by capillary electrophoresis on sodium dodecyl sulfate gel (SDS-CGE) - ISO/DIS 23293 - 06-19
- Evaluation and Recommendation...
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