5. Conclusion

This article describes the principles of capillary electrophoresis. Numerous parameters, such as pH, ionic strength, electrolyte composition and analysis temperature, can be used to modify the electrophoretic and electroosmotic mobilities, and therefore the velocities of compounds, in order to optimize their separation. Mastering these factors is the key to developing analysis methods that are not only effective in terms of efficiency, resolution and analysis time, but also reproducible and robust. The diversity of separation methods available in capillary electrophoresis means that this single technique can be used to access different and complementary information for a given sample. For example, in the field of biopharmaceutical compounds, where capillary electrophoresis is essential, it can be used to characterize purity and stability, as well as variants in charge and size, isoelectric...

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page Principle of the different separation modes in capillary electrophoresis

Glossary

Bibliography

(1) - PORRAS (S.P.), RIEKKOLA (M.-L.), KENNDLER (E.) - The principles of migration and dispersion in capillary zone electrophoresis in nonaqueous solvents. - Electrophoresis, 24, p. 1485-1498 (2003).
(2) - FRIEDL (W.), REIJENGA (J.C.),...

Norms and standards (non-exhaustive list)

[IDF 206:2006] Milk powder – Determination of soybean and pea proteins by capillary electrophoresis in the presence of sodium dodecyl sulfate (SDS-CE) – Screening method - ISO 17129 - 2006
Powdered milk-based infant formulas – Quantification of whey protein content by capillary electrophoresis on sodium dodecyl sulfate gel (SDS-CGE) - ISO/DIS 23293 - 06-19
Evaluation and Recommendation...