Overview
FrançaisABSTRACT
Air, surfaces and water are environmental media contaminated by a varied microbial flora derived from persons and the environment. Control of the microbial environment is designed to protect an industrial process, a product or a vulnerable patient. It requires assessment and control of the risk factors associated with this environment. Microbiological measurements of environmental media help monitor a controlled environment. This article describes the sampling and analysis methods for environmental microorganisms.
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Read the articleAUTHOR
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Fabien SQUINAZI: Medical biologist - Former Director, Laboratoire d'Hygiène de la Ville de Paris, Paris, France
INTRODUCTION
Environmental media (air, surfaces, water) are subject to microbiological contamination that is permanent but variable in time and space. The micro-organisms present are environmental saprophytes, commensals or human pathogens. As vectors of contamination, environmental media disseminate micro-organisms over varying distances, insidiously contributing to the progressive contamination of the environment's various inert supports. Some of these micro-organisms are the cause of product spoilage in various industries, and of healthcare-associated infections in vulnerable patients in healthcare establishments.
In the face of this ubiquitous contamination, the protection of vulnerable products and individuals can only be envisaged in a controlled microbial environment. It is therefore essential to assess the risks of contamination, and to manage these risks appropriately and consistently. The microbiological quality of all environmental media must be constantly controlled and regularly monitored. This monitoring is based on a variety of methods, both old and new, for sampling and analyzing environmental micro-organisms, which are presented in this article.
This article is part of a series of four dedicated to microbiology analysis:
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KEYWORDS
microorganisms | microbial flora | microbial contamination
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Laboratory quality and safety procedures
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Microbiology analysis
Bibliography
Standards and norms
- Cleanrooms and related controlled environments. Biocontamination control – Part 1: General principles and methods - NF EN ISO 14698-1 - 03-04
- Health care facilities – Controlled environment zones. Requirements for airborne contamination control - NF S90-351 - 04-13
- Indoor air – Part 16: Mould detection and enumeration – Filtration sampling - NF ISO 16000-16 - 2009
- Indoor air – Part 17: Mould detection...
Regulations
European Good Manufacturing Practice (EuGMP). Specific guideline: LD.1 Manufacture of sterile medicinal products
European Pharmacopoeia 0169. 8th edition. Specifications for water for injections
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