Article | REF: RE241 V1

Microfluidics and Tumor DNA - Contribution of digital PCR

Authors: Philippe NIZARD, Aurélie KROL, Pierre LAURENT-PUIG, Valérie TALY

Publication date: February 10, 2015

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


Overview

Français

ABSTRACT

Digital PCR (polymerase chain reaction) is a way to cross the technological gap in detection and quantification of rare DNA sequences, such as mutations found in tumors. Detection of these rare sequences is of high importance for improving cancer patient care. Their detection in tumors or in biological fluids such as plasma or urine could allow the adaptation of treatment to the precise molecular characteristics of the tumor, and limit risks of recurrence, thereby increasing patient survival. After a brief introduction of the research described, this article first discusses cancer and its biomarkers, then follows with the emergence of digital PCR, and lastly explores the perspectives and potential evolution of digital PCR.

Read this article from a comprehensive knowledge base, updated and supplemented with articles reviewed by scientific committees.

Read the article

AUTHORS

  • Philippe NIZARD: Research engineer at UMRS1147, Université Sorbonne Paris Cité, INSERM UMR-S1147, Centre universitaire des Saints-Pères, Paris, France

  • Aurélie KROL: Research engineer at UMRS1147, Université Sorbonne Paris Cité, INSERM UMR-S1147, Centre universitaire des Saints-Pères, Paris, France

  • Pierre LAURENT-PUIG: PU-PH, Director of UMRS1147, Université Sorbonne Paris Cité, INSERM UMR-S1147, center universitaire des Saints-Pères, Paris, France

  • Valérie TALY: CNRS Research Associate (CR1) - Head of the "Translational Research and Microfluidics" group, Sorbonne Paris Cité University, INSERM UMR-S1147, Saints-Pères University Center, Paris, France

 INTRODUCTION

Key points

Area: Genetic analysis

Degree of technology diffusion: Emergence | Growth | Maturity

Technologies involved: Microfluidics, digital PCR, PCR, quantitative PCR, molecular biology

Applications: Cancer biomarker research

Key French players: GDR Micro et Nano Fluidique

Contact: [email protected] ; [email protected]

You do not have access to this resource.

Exclusive to subscribers. 97% yet to be discovered!

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


The Ultimate Scientific and Technical Reference

A Comprehensive Knowledge Base, with over 1,200 authors and 100 scientific advisors
+ More than 10,000 articles and 1,000 how-to sheets, over 800 new or updated articles every year
From design to prototyping, right through to industrialization, the reference for securing the development of your industrial projects

This article is included in

Healthcare technologies

This offer includes:

Knowledge Base

Updated and enriched with articles validated by our scientific committees

Services

A set of exclusive tools to complement the resources

Practical Path

Operational and didactic, to guarantee the acquisition of transversal skills

Doc & Quiz

Interactive articles with quizzes, for constructive reading

Subscribe now!

Ongoing reading
Microfluidics and tumor DNA