Overview
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ABSTRACT
Digital PCR (polymerase chain reaction) is a way to cross the technological gap in detection and quantification of rare DNA sequences, such as mutations found in tumors. Detection of these rare sequences is of high importance for improving cancer patient care. Their detection in tumors or in biological fluids such as plasma or urine could allow the adaptation of treatment to the precise molecular characteristics of the tumor, and limit risks of recurrence, thereby increasing patient survival. After a brief introduction of the research described, this article first discusses cancer and its biomarkers, then follows with the emergence of digital PCR, and lastly explores the perspectives and potential evolution of digital PCR.
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Read the articleAUTHORS
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Philippe NIZARD: Research engineer at UMRS1147, Université Sorbonne Paris Cité, INSERM UMR-S1147, Centre universitaire des Saints-Pères, Paris, France
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Aurélie KROL: Research engineer at UMRS1147, Université Sorbonne Paris Cité, INSERM UMR-S1147, Centre universitaire des Saints-Pères, Paris, France
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Pierre LAURENT-PUIG: PU-PH, Director of UMRS1147, Université Sorbonne Paris Cité, INSERM UMR-S1147, center universitaire des Saints-Pères, Paris, France
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Valérie TALY: CNRS Research Associate (CR1) - Head of the "Translational Research and Microfluidics" group, Sorbonne Paris Cité University, INSERM UMR-S1147, Saints-Pères University Center, Paris, France
INTRODUCTION
Area: Genetic analysis
Degree of technology diffusion: Emergence | Growth | Maturity
Technologies involved: Microfluidics, digital PCR, PCR, quantitative PCR, molecular biology
Applications: Cancer biomarker research
Key French players: GDR Micro et Nano Fluidique
Contact: [email protected] ; [email protected]
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Microfluidics and tumor DNA
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