4. Conclusion

As we have seen, intravital two-photon fluorescence microscopy is a highly interesting imaging tool, providing access to microvascularization parameters in cerebrovascular imaging. These microscopic data, inaccessible to conventional imaging methods (MRI, CT-scan), are an essential complement to the macroscopic data provided by the latter, with a view to establishing a complete mapping of the brain. Although the depths of observation achievable with these methods are currently limited to a few hundred microns, advances in instrumentation should eventually make it possible to extend this limit. A team from Cornell University recently obtained images of the vascularization of the cortex of a mouse to a depth of 1.4 mm, using a laser source capable of exciting a three-photon fluorophore at 1,675 nm

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Bibliography

(1) - KIM (S.G.) - Perfusion MR imaging : evolution from initial development to functional studies. - Neuroimage, 62(2), p. 672-675 (2012).
(2) - KLEINFELD (D.), MITRA (P.P.), HELMCHEN (F.), DENK (W.) - Fluctuations and stimulus-induced changes in blood...

Software tools

Free software for image processing and analysis:

ImageJ http://rsb.info.nih.gov/ij/

FIJI image http://fiji.sc/Fiji

Websites

http://neurosciences.ujf-grenoble.fr/main-plateformes-4-sub-plate-forme-de-microscopie---microscopie-biphotonique-22-lang-fr.html

http://www.chu-grenoble.fr/doc/Documents/clinatec%20presse%283%29.pdf

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Events

Spie Photonics West World Congress on (Bio)Photonic Imaging: this congress is held every year on the east coast of America ( http://spie.org/x2584.xml ). The European variant SPIE Photonics Europe http://spie.org/x12290.xml

École thématique Microscopie...

Standards and norms

Laser safety – Part 1: Equipment classification, specifications and user guide - EN 60825-1/A2 -

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Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes.

Arrêté du 1 ^er février 2013 fixant les conditions d'agrément, d'aménagement et de fonctionnement des établissements utilisateurs, éleveurs ou fournisseurs d'animaux utilisés à des fins scientifiques et leurs...