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2. Cerebrovascular imaging devices using two-photon microscopy
2.1 Two-photon microscope
As mentioned above, one of the most relevant devices for intravital microscopy is the two-photon microscope. In biomedical research and preclinical animal studies, this microscope consists of an upright stand, a pulsed infrared (IR) laser source and an IR-optimized scanner (figure 2 ). Unlike its confocal counterpart, the biphotonic nature of the excitation means that a pin-hole diaphragm is not required to select the fluorescence emitted at the focal plane of the objective. The five main manufacturers of multiphoton microscopes are Zeiss (LSM 7MP), Olympus (Fluoview ®), Nikon (A1R MP), Leica (TCS MP5, TCS SP8 MP) and LaVision BioTec...
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Cerebrovascular imaging devices using two-photon microscopy
Two-photon fluorescent probes for intravital and cerebrovascular imaging
Bibliography
Software tools
Free software for image processing and analysis:
ImageJ http://rsb.info.nih.gov/ij/
FIJI image http://fiji.sc/Fiji
Events
Spie Photonics West World Congress on (Bio)Photonic Imaging: this congress is held every year on the east coast of America ( http://spie.org/x2584.xml ). The European variant SPIE Photonics Europe http://spie.org/x12290.xml
École thématique Microscopie...
Standards and norms
- Laser safety – Part 1: Equipment classification, specifications and user guide - EN 60825-1/A2 -
Regulations
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes.
Arrêté du 1 er février 2013 fixant les conditions d'agrément, d'aménagement et de fonctionnement des établissements utilisateurs, éleveurs ou fournisseurs d'animaux utilisés à des fins scientifiques et leurs...
Patents
MONNEREAU (C.), ANDRAUD (C.), MAURY (O.). – Novel water-soluble chromophore. PCT Int. Appl. WO 2013011242 A1 20130124 (2013).
Directory
Manufacturers – Suppliers – Distributors (non-exhaustive list)
Microscopes :
Zeiss http://microscopy.zeiss.com/microscopy/en_de/products/confocal- microscopes/lsm-7-mp.html
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