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Article | REF: MED500 V1

3D visualization of the cerebral vasculature by two-photon microscopy

Authors: Florence APPAIX, Cyrille MONNEREAU, Boudewijn VAN DER SANDEN

Publication date: August 10, 2014 | Lire en français

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    2. Cerebrovascular imaging devices using two-photon microscopy

    2.1 Two-photon microscope

    As mentioned above, one of the most relevant devices for intravital microscopy is the two-photon microscope. In biomedical research and preclinical animal studies, this microscope consists of an upright stand, a pulsed infrared (IR) laser source and an IR-optimized scanner (figure 2 ). Unlike its confocal counterpart, the biphotonic nature of the excitation means that a pin-hole diaphragm is not required to select the fluorescence emitted at the focal plane of the objective. The five main manufacturers of multiphoton microscopes are Zeiss (LSM 7MP), Olympus (Fluoview ®), Nikon (A1R MP), Leica (TCS MP5, TCS SP8 MP) and LaVision BioTec...

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