Article | REF: MED500 V1

3D visualization of the cerebral vasculature by two-photon microscopy

Authors: Florence APPAIX, Cyrille MONNEREAU, Boudewijn VAN DER SANDEN

Publication date: August 10, 2014

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3. Two-photon fluorescent probes for intravital and cerebrovascular imaging

3.1 Two-photon excitable fluorescent probes

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3.1.1 General background/spectroscopic criteria

Like most modern biomedical imaging techniques, two-photon optical microscopy requires the use of molecular contrast agents, in this case fluorescent probes capable of efficiently absorbing two photons simultaneously. The mechanisms of nonlinear optics in general, and of multiphoton absorption processes in particular, correspond to complex physical processes whose theoretical underpinnings go far beyond the scope of this article....

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Two-photon fluorescent probes for intravital and cerebrovascular imaging

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Conclusion

Bibliography

(1) - KIM (S.G.) - Perfusion MR imaging : evolution from initial development to functional studies. - Neuroimage, 62(2), p. 672-675 (2012).
(2) - KLEINFELD (D.), MITRA (P.P.), HELMCHEN (F.), DENK (W.) - Fluctuations and stimulus-induced changes in blood...

Software tools

Free software for image processing and analysis:

ImageJ http://rsb.info.nih.gov/ij/

FIJI image http://fiji.sc/Fiji

Websites

http://neurosciences.ujf-grenoble.fr/main-plateformes-4-sub-plate-forme-de-microscopie---microscopie-biphotonique-22-lang-fr.html

http://www.chu-grenoble.fr/doc/Documents/clinatec%20presse%283%29.pdf

...

Events

Spie Photonics West World Congress on (Bio)Photonic Imaging: this congress is held every year on the east coast of America ( http://spie.org/x2584.xml ). The European variant SPIE Photonics Europe http://spie.org/x12290.xml

École thématique Microscopie...

Standards and norms

Laser safety – Part 1: Equipment classification, specifications and user guide - EN 60825-1/A2 -

Regulations

Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes.

Arrêté du 1 ^er février 2013 fixant les conditions d'agrément, d'aménagement et de fonctionnement des établissements utilisateurs, éleveurs ou fournisseurs d'animaux utilisés à des fins scientifiques et leurs...