11. Conclusion
The crystallography of biological macromolecules occupies a central place in modern biology, as the three-dimensional structure of a macromolecule or macromolecular complex sheds new and original light that is essential for understanding biological function. In most cases, this is a revolution, and several Nobel Prizes have crowned the most outstanding successes. Purification of the macromolecule(s) and obtaining quality crystals (diffraction limit better than 3 Å) are still two limiting stages in any structural project. What's more, solving the phase problem is still in some cases a long and tedious process, particularly for large macromolecular edifices. In simple cases, 3D structures are routinely determined from a very limited number of crystals, sometimes of very small sizes (a few micrometers), with a minimum of human intervention. By simple cases we mean macromolecules which, given...
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Conclusion
Bibliography
Bibliography
Websites
Integrative structural biology
on a European scale, Instruct-ERIC https://www.structuralbiology.eu
a French infrastructure, FRISBI http://frisbi.eu
Biocrystallography reference...
Software tools
Data collection :
• HKL2000/HKL3000, MOSFLM, XDS
• expert systems: xia2, autoproc
Phasing MIR, MAD, SAD
• SHARP, SOLVE, CRANK2,SHELX
Molecular replacement :
• Phaser, Molrep, AMoRe, ARCIMBOLDO
• expert systems: MrBUMP, BALBES, MoRDa, AMPLE, SIMBAD...
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