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4. Regeneration of biosensors
For routine use, a biosensor must be reusable. The analyte must therefore be detached from the ligand under chemical conditions that enable it to retain its full capture capacity. In the case of immunosensors, the higher the affinity between the antibody and its respective antigen, the greater the analytical sensitivity of the system. Regeneration of the antibody binding sites immobilized on the biosensor surface will then require the use of drastic conditions. Regeneration using acidic or alkaline solutions, guanidinium chloride or a sudden change in ionic strength may reduce the ability of the antibody to capture the antigen, leading to a gradual decrease in measured concentrations and therefore a reduction in the sensor's lifetime.
Furthermore, very short reaction times between antibody and analyte in continuous flow systems can result in increased cross-reactivity...
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Regeneration of biosensors
Main types of biosensors
Economic data
The biosensor industry is booming. The market comprises 4 segments: medical, environmental, agri-food and military, with medical applications accounting for the vast majority (90% of sales are of glucose-detecting biosensors).
In the food industry, pathogen detection is the biggest market. In the USA, a recent study
References
Commercially available biosensors
Despite a large number of publications on biosensors applied to food analysis, only a small number of systems are commercially available. The disadvantages that need to be overcome are the limited lifetime of biological compounds, large-scale production and ease of use.
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Few biosensors are currently used in the dairy industry for on-line analysis, although...
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