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2. Optical devices
This chapter covers the principle of epifluorescence microscopy, as well as the necessary ancillary devices such as excitation sources, and technical solutions to the constraints associated with the observation of biological material.
2.1 Full-field microscope
Fluorescence microscopes have come to be known as "widefield" or "full-field" microscopes, to distinguish them from the confocal microscopes that have become widespread since the 1990s.
The fluorescence microscope is based on the classic visible light microscope (optical principles are described in
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Mechanical and dimensional measurements
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Optical devices
Techniques for observing thick objects
Bibliography
Software tools
Image capture and peripheral control
Metamorph http://www.moleculardevices.comcom/Products/Software/Meta-Imaging-Series/MetaMorph.html
Peripheral control
Websites
A wealth of information about microscopy can be found on the website published by the Federal University of Florida. http://www.micro.magnet.fsu.edu/
Historical and review articles on light microscopy from the journal Nature http://www.nature.com/milestones/light-microscopy
...Events
Thematic school on functional microscopy in biology http://www.mifobio.fr/
Directory
Manufacturers – Microscopes (non-exhaustive list)
Leica http://www.leica-microsystems.com/
Nikon http://www.nikoninstruments.com/
Olympus http://www.olympusmicro.com/
...
The Ultimate Scientific and Technical Reference
