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4. Advanced microscopy techniques
In this section, we'll focus on microscopic measurements of fluorescence intensity, which constitute the techniques of fluorescence imaging. Measurements of other physical parameters of fluorescence, such as lifetime, dichroism, etc., will not be discussed.
4.1 Restricting the area explored
In this section, we will describe specific microscopy techniques for limiting the size of the "blur" zone created by a fluorescent source. The causes of blur are mainly due to the illumination of fluorescent objects outside the plane of focus and to the image spreading function of a point source ("PSF"). The various confocal microscopes and TIRF described below improve the first situation, while super-resolution techniques "restrict" the PSF. These restrictions improve...
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Advanced microscopy techniques
Image sensors
Bibliography
Software tools
Image capture and peripheral control
Metamorph http://www.moleculardevices.comcom/Products/Software/Meta-Imaging-Series/MetaMorph.html
Peripheral control
Websites
A wealth of information about microscopy can be found on the website published by the Federal University of Florida. http://www.micro.magnet.fsu.edu/
Historical and review articles on light microscopy from the journal Nature http://www.nature.com/milestones/light-microscopy
...Events
Thematic school on functional microscopy in biology http://www.mifobio.fr/
Directory
Manufacturers – Microscopes (non-exhaustive list)
Leica http://www.leica-microsystems.com/
Nikon http://www.nikoninstruments.com/
Olympus http://www.olympusmicro.com/
...
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