Article | REF: P1474 V1

Planar chromatography -Part 2

Authors: Antoine Michel SIOUFFI, Chantal DAUPHIN, Dominique PRADEAU

Publication date: March 10, 2007, Review date: November 1, 2022

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1. Visual detection

Intended for qualitative analysis, visual detection is widely used in the analysis of narcotics, for example. In all cases, the plate is illuminated by a suitable light source:

  • white light is used to identify coloured substances;

  • ultraviolet light enables fluorescent substances to be seen when illuminated at these wavelengths.

To facilitate detection, TLC plate manufacturers mix a fluorescent indicator into the adsorbent layer; this may be an inorganic salt that gives the plate a yellow (uranyl acetate) or yellow-green (zinc silicate) color. The most widely used is a mixed sulfide of zinc and cadmium, fluorescing at 254 nm, hence the name F 254.

Organic salts generally fluoresce at 366 nm; the most commonly used are 3-hydroxypyrene 5,8,10-trisulfonate...

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