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Quizzed article | REF: P3872 V2

HPLC-ICP-MS coupling and application to speciation

Authors: Véronique VACCHINA, Martine POTIN-GAUTIER, Fabienne SEBY

Publication date: April 10, 2019, Review date: September 2, 2020

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2. Instrumentation

2.1 HPLC

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2.1.1 Principle

High-performance liquid chromatography (HPLC) is a technique for separating compounds in a mixture by partitioning between a liquid mobile phase and a stationary phase.

Upstream of an HPLC unit is a reservoir for feeding a pump with mobile phase (pure solvent or solvent mixture) for several hours. The mobile phase is generally degassed and filtered to avoid the risk of de-priming and/or clogging. The pump delivers a constant...

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