2. Instrumentation

2.1 HPLC

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2.1.1 Principle

High-performance liquid chromatography (HPLC) is a technique for separating compounds in a mixture by partitioning between a liquid mobile phase and a stationary phase.

Upstream of an HPLC unit is a reservoir for feeding a pump with mobile phase (pure solvent or solvent mixture) for several hours. The mobile phase is generally degassed and filtered to avoid the risk of de-priming and/or clogging. The pump delivers a constant...

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Instrumentation

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Application to speciation analysis

Bibliography

(1) - TEMPLETON (D.M.), FUJISHIRO (H.) - Terminology of elemental speciation – An IUPAC perspective. - Coordination Chemistry Reviews, 352, p. 424-431 (2017).
(2) - SIMON (S.), LOBOS (G.), PANNIER (F.), DE GREGORI (I.), PINOCHET (H.), POTIN-GAUTIER (M.) -...

Standards and norms

Food products – Determining elements and their chemical forms – Determination of inorganic arsenic content in food products of marine and plant origin, by HPLC with anion exchange and inductively coupled plasma mass spectrometry (ICP-MS) - NF EN 16802 - 2016
Water quality – Determination of arsenic chemical forms (III) and (V) – Method using high-performance liquid chromatography (HPLC) with detection by plasma...

Regulations

European Commission Regulation (EC) No 2015/1006 of 25 June 2015 amending Regulation (EC) No 1881/2006 as regards maximum levels for inorganic arsenic in foodstuffs setting maximum levels for certain contaminants in foodstuffs (OJ L. 161, 26/6/2015, p. 14-16)

Directive 2000/53/EC of the European Parliament and of the Council of 27 January 2003 on end-of-life vehicles (OJ L. 269 of 21/10/2000, pp. 34-43)...