Article | REF: J2787 V1

Centrifugal partition chromatography Operating conditions, modeling and scale-up

Authors: Luc MARCHAL, Jean-Hugues RENAULT, Sébastien CHOLLET

Publication date: March 10, 2017

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


Overview

Français

ABSTRACT

Centrifugal partition chromatography is a separation process based on the distribution of solute between two immiscible liquid phases. The absence of solid support lends this separation technique large capacity and versatility, making it convenient for delicate separations. Operation modes can take advantage of the fluid nature of the stationary phase. This article gives a synthesis of the available knowledge on the hydrodynamics of stationary and mobile phases and process modelling with mass transfer kinetics to optimise the separation (productivity, yield) and transpose it to a larger scale.

Read this article from a comprehensive knowledge base, updated and supplemented with articles reviewed by scientific committees.

Read the article

AUTHORS

  • Luc MARCHAL: Senior Lecturer HDR Process Engineering Laboratory – environment – agroalimentaire UMR CNRS 6144 – University of Nantes, Saint Nazaire, France

  • Jean-Hugues RENAULT: Professor Institut de chimie moléculaire de Reims UMR CNRS 7312 – University of Reims Champagne-Ardenne, Reims, France

  • Sébastien CHOLLET: Doctor CPC Eng – Capacités SAS, Nantes, France

 INTRODUCTION

Centrifugal Partition Chromatography (CPC) is a multi-stage separation technique using compact columns consisting of a cascade of chambers or partition cells linked by channels and subjected to a centrifugal acceleration field. The separation mechanism is based on the difference in compound distribution between two immiscible liquid phases. In the CPC column, one of the liquids is kept stationary by the rotation of the column, without the need for a solid support, while the mobile phase passes through the stationary phase at a flow rate dictated by a pump. The CPC chain and its range of applications are similar to those of a preparative HPLC (high-performance liquid chromatography) chain.

The main developments in CPC took place in the 1990s and 2000s with the emergence of new equipment manufacturers, bringing the technology to a level of maturity that makes it industrializable (columns are available in different materials, for volumes ranging from 30 mL to 25 L). The absence of a solid support for capture or fractionation operations means that complex feedstocks can be processed, as is often the case with natural substances (complex secondary metabolites, for example) or biotechnology-derived substances, without the risk of overloading or clogging the support (resin, silica). This operating flexibility, the low cost of the stationary phase and the reduced consumption of solvents mean that CPC can be used to extend the range of applications for liquid chromatography and reduce the number of purification stages. The purification of high value-added natural substances (peptides, alkaloids) was initially used as a case study. Although scale-up has long been considered a linear transposition, it is shown here that CPC is controlled by :

  • phase hydrodynamics and material transfer ;

  • column geometry, according to non-linear rules.

The CPC column modeling and engineering tools presented in this article can be used to size columns for a desired application and productivity, or to transpose a separation from R&D equipment to production equipment.

Like all processes based on the transfer of matter between two fluids, the performance of a CPC column (efficiency, resolution, productivity) results from the hydrodynamics of the phases, which are presented first.

The models developed can be used to describe and predict chromatograms in both elution and displacement modes. The models therefore incorporate the transport, transfer and, where applicable, reaction terms of the species involved in the separation. Phase hydrodynamics are taken into account in the models. via changes in phase ratio, phase velocity...

You do not have access to this resource.

Exclusive to subscribers. 97% yet to be discovered!

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


The Ultimate Scientific and Technical Reference

A Comprehensive Knowledge Base, with over 1,200 authors and 100 scientific advisors
+ More than 10,000 articles and 1,000 how-to sheets, over 800 new or updated articles every year
From design to prototyping, right through to industrialization, the reference for securing the development of your industrial projects

This article is included in

Unit operations. Chemical reaction engineering

This offer includes:

Knowledge Base

Updated and enriched with articles validated by our scientific committees

Services

A set of exclusive tools to complement the resources

Practical Path

Operational and didactic, to guarantee the acquisition of transversal skills

Doc & Quiz

Interactive articles with quizzes, for constructive reading

Subscribe now!

Ongoing reading
Centrifugal partition chromatography