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1. Fluorescent preparations
1.1 The physical principle of molecular fluorescence
Light interacts with a fluorescent molecule by absorbing a photon, which transfers its energy to a peripheral electron. This electron then restores part of the acquired energy in the form of light radiation. These are the well-known phenomena of fluorescence and phosphorescence, described in the literature (
The prediction of the wavelengths capable of exciting a fluorescent molecule, and the wavelengths of photon emission upon de-excitation, is made using Jablonski diagrams, which summarize the energetic transitions...
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Fluorescent preparations
Optical devices
Bibliography
Software tools
Image capture and peripheral control
Metamorph http://www.moleculardevices.comcom/Products/Software/Meta-Imaging-Series/MetaMorph.html
Peripheral control
Websites
A wealth of information about microscopy can be found on the website published by the Federal University of Florida. http://www.micro.magnet.fsu.edu/
Historical and review articles on light microscopy from the journal Nature http://www.nature.com/milestones/light-microscopy
...Events
Thematic school on functional microscopy in biology http://www.mifobio.fr/
Directory
Manufacturers – Microscopes (non-exhaustive list)
Leica http://www.leica-microsystems.com/
Nikon http://www.nikoninstruments.com/
Olympus http://www.olympusmicro.com/
...
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