Français
1. From biological samples to proteins
1.1 Extraction and solubilization
Whatever the proteomic study to be carried out, sample preparation is by far the most important stage in the analysis. All the proteins in the sample must not only be extracted, but also soluble, without their interaction partners (protein/DNA, protein/RNA, metabolite, etc.). Moreover, for two-dimensional electrophoresis, proteins must remain soluble close to their isoelectric point (pI). It is also clear that extraction and solubilization must be reproducible. Several approaches are described in the literature
The Ultimate Scientific and Technical Reference
This article is included in
Analysis and Characterization
This offer includes:
Knowledge Base
Updated and enriched with articles validated by our scientific committees
Services
A set of exclusive tools to complement the resources
Practical Path
Operational and didactic, to guarantee the acquisition of transversal skills
Doc & Quiz
Interactive articles with quizzes, for constructive reading
From biological samples to proteins
Protein purification
Bibliography
The Ultimate Scientific and Technical Reference
