3. Implication of capillary electrophoresis in the study of enzymatic reactions

CE was first used in 1991 by Banke et al. to monitor alkaline protease activity. Since then, CE has been developed for the determination of enzyme kinetics and the identification of substrates, activators and inhibitors of numerous enzymes. The different approaches found in the literature for the study of enzymatic reactions in CE are shown in figure 1 . Two strategies can be distinguished, depending on whether incubation takes place outside or inside the capillary...

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Implication of capillary electrophoresis in the study of enzymatic reactions

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Bibliography

(1) - NEHME (H.), NEHME (R.), LAFITE (P.) et al - In-capillary reactant mixing for monitoring glycerol kinase kinetics by CE. - J. Sep. Sci., 36(13), p. 2151-2157 (2013).
(2) - NØRBY (J.G.), ESMANN (M.) - The effect of ionic strength and specific anions on...