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Article | REF: P1455 V1

Liquid chromatography - Separation theory and methods

Authors: Marcel CAUDE, Alain JARDY

Publication date: October 10, 1994

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2. Kinetics

2.1 Reduced sizes

In plateau theory, the HEPT deduced from the variance σ 2 of the elution peak : H=LN=Lσ2tR2

can only be seen as a global measure of the influence of all the experimental parameters involved, but it doesn't allow us to specify the contribution of each parameter to the spreading of the peak as it progresses through the column. Spread depends on :

  • the diameter and nature of the particles...

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