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Quizzed article | REF: P3367 V2

Capillary electrophoresis - Applications

Author: Nathalie DELAUNAY

Publication date: September 10, 2021, Review date: January 6, 2023 | Lire en français

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    3. Neutral organic compounds

    3.1 Separation in EKC mode

    CE is well known for separating charged compounds, but it is also perfectly suited to neutral organic compounds, in particular with its EKC mode, which is based on the introduction into the separation electrolyte of an additive capable of developing interactions with the compounds to be analyzed, playing the role of a pseudo-stationary phase.

    • This is particularly true of MEKC, where neutral compounds are separated solely on the basis of their hydrophobicity. Indeed, they all have zero electrophoretic mobilities and migrate at the same speed when in the separating electrolyte, that of the electroosmotic flow. On the other hand, when they interact with micelles by penetrating their hydrophobic cores, they migrate...

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