3. Neutral organic compounds

3.1 Separation in EKC mode

CE is well known for separating charged compounds, but it is also perfectly suited to neutral organic compounds, in particular with its EKC mode, which is based on the introduction into the separation electrolyte of an additive capable of developing interactions with the compounds to be analyzed, playing the role of a pseudo-stationary phase.

This is particularly true of MEKC, where neutral compounds are separated solely on the basis of their hydrophobicity. Indeed, they all have zero electrophoretic mobilities and migrate at the same speed when in the separating electrolyte, that of the electroosmotic flow. On the other hand, when they interact with micelles by penetrating their hydrophobic cores, they migrate...

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Neutral organic compounds

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Bibliography

(1) - BECKERS (J.L.), BOCEK (P.) - The preparation of background electrolytes in capillary zone electrophoresis: golden rules and pitfalls. - Electrophoresis, 24, 518-535 (2003).
(2) - JOHNS (C.), BREADMORE (M.C.),...

Standards and norms

Milk powder – Determination of soybean and pea proteins by capillary electrophoresis in the presence of sodium dodecyl sulfate (SDS-CE) – Screening method - ISO 17129 - 2006
Powdered milk-based infant formulas – Quantification of whey protein content by capillary electrophoresis on sodium dodecyl sulfate gel (SDS-CGE) - ISO/DIS 23293 - 2020
Evaluation and Recommendation of Pharmacopoeial Texts for Use in...