5. Amino acids
AA amino acids all have, as their name suggests, a carboxylic acid function and an amine function in their structure, and may differ in the position of the amine function relative to the carboxyl group, or in the grouping present on their side chain, which may also have acid-base properties.
They can therefore be ionized and separated into CZE. However, one of the difficulties in their analysis lies in the fact that very few AAs possess a chromophore group. Most are therefore UV-transparent and non-fluorescent, with the exception of tyrosine, tryptophan and phenylalanine.
While this does not pose a problem for direct MS detection
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Amino acids
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Standards and norms
- Milk powder – Determination of soybean and pea proteins by capillary electrophoresis in the presence of sodium dodecyl sulfate (SDS-CE) – Screening method - ISO 17129 - 2006
- Powdered milk-based infant formulas – Quantification of whey protein content by capillary electrophoresis on sodium dodecyl sulfate gel (SDS-CGE) - ISO/DIS 23293 - 2020
- Evaluation and Recommendation of Pharmacopoeial Texts for Use in...
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