2. Organic ions

2.1 Separation in CZE mode

As for the analysis of organic ions in CE, as with inorganic ions, the presence of a charge gives them their own electrophoretic mobility, which enables them to be separated in CZE according to the charge/size criterion. Approaches are generally distinct for cations and anions, with the aim of achieving rapid analyses, i.e. to place as far as possible in a co-electroosmotic mode, and high performance by optimizing the differences in mobility between analytes. Unlike inorganic ions, where complexation is often used, this approach is very rarely possible for organic ions, and selectivity depends on the classical CZE parameters, i.e. pH, ionic strength and the nature and solvent content of the separating electrolyte.

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Organic ions

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Neutral organic compounds

Bibliography

(1) - BECKERS (J.L.), BOCEK (P.) - The preparation of background electrolytes in capillary zone electrophoresis: golden rules and pitfalls. - Electrophoresis, 24, 518-535 (2003).
(2) - JOHNS (C.), BREADMORE (M.C.),...

Standards and norms

Milk powder – Determination of soybean and pea proteins by capillary electrophoresis in the presence of sodium dodecyl sulfate (SDS-CE) – Screening method - ISO 17129 - 2006
Powdered milk-based infant formulas – Quantification of whey protein content by capillary electrophoresis on sodium dodecyl sulfate gel (SDS-CGE) - ISO/DIS 23293 - 2020
Evaluation and Recommendation of Pharmacopoeial Texts for Use in...