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3. Technical prerequisites for fluorescence microscopy
3.1 Laser and spectral bands of interest in fluorescence microscopy
In optical microscopy, the choice of laser source and its physical parameters is conditioned by the linear or non-linear excitation process to be used, and by the fluorophore involved. In linear fluorescence microscopy, it is common to find 3, 4 or even 5 different laser sources for a single confocal microscope . In multiphoton microscopy, the standard system uses a single laser source. To define the most suitable laser technology for a given application - linear or non-linear...
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Technical prerequisites for fluorescence microscopy
Laser sources developed for MMP
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Linear absorption spectra of the most common fluorophores can be found by following one of the links below:
Thermo Fisher Scientific : http://www.thermofisher.com › home › labeling-chemistry
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