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4. Quantification
4.1 Differential quantification
4.1.1 Isotopic metabolic labeling
The metabolic isotope labelling technique is based on the use of modified culture media incorporating labelled amino acids in place of native amino acids. As a result, proteins derived from cells grown on these modified culture media incorporate the tagged amino acids in their sequences. As a result, this technique limits its field of application to proteins from cultured cells. Isotope incorporation methodology was first presented in 2002 by Matthias...
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