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2. Meta-omics techniques: principles, methodologies and applications
Since the 1980s, the analysis of soil microbial communities has seen the emergence of a new paradigm (figure 5 ). Pasteurian analyses, based on the characterization of pure strains grown in the laboratory, have given way to approaches based on the characterization of biological material, notably DNA, but also other biological sources such as RNA, proteins or metabolites. This material is now extracted directly from an environmental sample, making it possible to move on from the study of an isolated micro-organism to the analysis of microbial communities in their natural environment, known as "meta-omics" (figure
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Meta-omics techniques: principles, methodologies and applications
The contribution of meta-omics to soil quality assessment
Bibliography
Websites
ISO standards : http://www.iso.org/committee/54366/x/catalogue/
AgrInnov project : http://revue-sesame-inra.fr/agrinnov-reva-un-reseau-pour-experimenter-de-bonnes-pratiques-agricoles-3/
...Standards and norms
- Soil quality – Sampling – Part 1: Guidelines for establishing sampling programs. - ISO 10381-1 - 2002
- Soil quality – Method for directly extracting DNA from soil samples. - ISO 11063 - 2012
- Soil quality – Estimation of microbial gene sequence abundance by quantitative polymerase chain reaction (PCR) amplification from DNA extracted directly from soil. - ISO 17601 - 2016
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Agrosolutions : http://www.agrosolutions.com/
Ambion (distributed by ThermoFisher) : http://www.thermofisher.com/fr/fr/home/brands/invitrogen/ambion/ambion-product-list.html
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