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2. PCR-based molecular methods
Most molecular techniques for detecting bacteria of interest are based on the polymerase chain reaction (PCR) method. Conceived in the early 1980s, PCR revolutionized experimental approaches in molecular biology. In the 1990s, it began to be used in diagnostics for the detection and identification of pathogenic bacteria. The development of real-time quantitative PCR (qPCR) and, more recently, digital PCR (dPCR) has made it possible to reliably diagnose and quantify pathogens in food. Many kits on the market today can detect the DNA of several pathogenic organisms of interest in a single reaction.
In vitro DNA amplification is a polymerase chain reaction (PCR) based on the complementary base pairing property of the two strands of the DNA molecule.
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PCR-based molecular methods
Isothermal amplification processes
Bibliography
Bibliography
Standards and norms
- Méthodes horizontales dans le domaine de l'analyse microbiologique de la chaîne alimentaire, du stade de production primaire aux produits pour l'alimentation humaine et animale, y compris l'environnement de la production et de la manutention des produits alimentaires - ISO/TC 34/SC 9 - 2016
- Food microbiology...
AFNOR French follow-up: Commission V08B "Microbiologie des aliments" (Food microbiology)
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