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4. Detection of TBT by the bioluminescent bacterium Escherichia coli TBT3
The idea was to develop a bacterium whose bioluminescence would be enhanced (induction) in the presence of a pollutant such as TBT.
4.1 Construction of the bacteria
Bioluminescence is the natural production of light by living organisms. These organisms include a number of bacterial species, most of them marine. The reaction is catalyzed by the enzyme luciferase. In the presence of oxygen, this enzyme catalyzes the oxidation of a long-chain aldehyde and reduced flavin mononucleotide (FMNH 2 ) to fatty acid and FMN. This reaction is accompanied by the emission of light at 490 nm and 590 nm as shown in figure
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Detection of TBT by the bioluminescent bacterium Escherichia coli TBT3
Conclusion
Bibliography
Standards and norms
- Water quality – Determination of the inhibitory effect of water samples on the luminescence of Vibrio fischeri (bioluminescent bacteria assay) – Part 3: method using freeze-dried bacteria (classification index: T90-320-3) - NF EN ISO 11348-3 - 02-99
- Soil quality – Determination of selected organotin compounds – Gas chromatography method - ISO 23161 - 2009
Regulations
Water Framework Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy (Official Journal L327 of 22/12/2000) – Annex VIII.
IMO, 2005, Antifouling Systems. International Convention on the control of harmful antifouling System on Ships. International Maritime Organization. London.
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