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5. Next-generation sequencing methods
5.1 Next-generation sequencing
In recent years, next-generation sequencing (NGS) methods combined with powerful bioinformatics approaches have revolutionized food microbiology. These technologies are developing rapidly, with ongoing improvements and ever-decreasing costs. In food microbiology, NGS methods are used in two main ways.
The first involves determining the complete genome sequence of a single bacterial colony in culture. Determining the complete genome sequence is highly resolvable compared with PCR-based detection systems. It also enables the precise typing of bacterial strains, and greatly facilitates the detection of food-borne disease outbreaks. Recent studies have demonstrated its application for Salmonella enterica serovar Enteritidis...
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Next-generation sequencing methods
Bibliography
Bibliography
Standards and norms
- Méthodes horizontales dans le domaine de l'analyse microbiologique de la chaîne alimentaire, du stade de production primaire aux produits pour l'alimentation humaine et animale, y compris l'environnement de la production et de la manutention des produits alimentaires - ISO/TC 34/SC 9 - 2016
- Food microbiology...
AFNOR French follow-up: Commission V08B "Microbiologie des aliments" (Food microbiology)
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The Ultimate Scientific and Technical Reference