Article | REF: RE281 V1

Molecular techniques for detection of foodborne bacteria

Authors: Jasmina VIDIC, Sandrine AUGER

Publication date: December 10, 2019, Review date: January 29, 2021

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


Français

3. Isothermal amplification processes

Due to the need to heat samples during PCR cycles, conventional PCR-based methods are not suitable for the development of portable point-of-care diagnostic kits. This problem is solved by isothermal amplification strategies, which are performed at a constant temperature. These methods share with PCR the specific amplification of one or more molecular markers (DNA) by a DNA polymerase. Isothermal amplification covers a wide range of methods . The most widely used for pathogen detection are LAMP (Loop-Mediated Amplification) and RPA...

You do not have access to this resource.

Exclusive to subscribers. 97% yet to be discovered!

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


The Ultimate Scientific and Technical Reference

A Comprehensive Knowledge Base, with over 1,200 authors and 100 scientific advisors
+ More than 10,000 articles and 1,000 how-to sheets, over 800 new or updated articles every year
From design to prototyping, right through to industrialization, the reference for securing the development of your industrial projects

This article is included in

Analysis and Characterization

This offer includes:

Knowledge Base

Updated and enriched with articles validated by our scientific committees

Services

A set of exclusive tools to complement the resources

Practical Path

Operational and didactic, to guarantee the acquisition of transversal skills

Doc & Quiz

Interactive articles with quizzes, for constructive reading

Subscribe now!

Ongoing reading
Isothermal amplification processes