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Article | REF: P1496 V1

The basics in Centrifugal Partition Chromatography

Author: Karine FAURE

Publication date: September 10, 2016

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4. Fields of application

4.1 CPC: a preparative technique

We have already seen the technological specifics of using a liquid stationary phase in chromatography. As far as the sample is concerned, the liquid nature of the stationary phase also offers two major advantages:

  • a very high loading capacity, as column overloading is often only related to the solubility limit of the compound in the phases. On a column of the same volume, it is common to inject a quantity 2 to 10 times greater in CPC than in HPLC;

  • the guarantee of no sample loss. There is no non-reversible adsorption on the stationary phase or possible cross-contamination, and if a compound has too high or infinite retention, it can be extruded with the liquid stationary...

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