Article | REF: BIO7200 V1

Biomedical fluorescence microscopy

Authors: Léon ESPINOSA, Yves TOURNEUR

Publication date: March 10, 2015

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


Français

5. Image sensors

In fluorescence, light levels are low, and sensor efficiency and noise play a significant role in the final image. Depending on the technology used, the image will be acquired point by point, line by line, or, more often, by direct filling of a CCD-type matrix sensor.

Sensors are characterized by their quantum efficiency and noise level. Efficiency is measured in % of electrons per photon. It depends on wavelength, and can approach 95%.

Thermal noise is the stochastic production of electrons in the absence of a light source. This noise is roughly doubled every 8°C. Sensors are cooled either to a temperature slightly above 0°C, or to a lower one, down to – 90 °C , taking into account the risk of icing and condensation (dry air blower, for example).

On cameras, read noise...

You do not have access to this resource.

Exclusive to subscribers. 97% yet to be discovered!

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


The Ultimate Scientific and Technical Reference

A Comprehensive Knowledge Base, with over 1,200 authors and 100 scientific advisors
+ More than 10,000 articles and 1,000 how-to sheets, over 800 new or updated articles every year
From design to prototyping, right through to industrialization, the reference for securing the development of your industrial projects

This article is included in

Analysis and Characterization

This offer includes:

Knowledge Base

Updated and enriched with articles validated by our scientific committees

Services

A set of exclusive tools to complement the resources

Practical Path

Operational and didactic, to guarantee the acquisition of transversal skills

Doc & Quiz

Interactive articles with quizzes, for constructive reading

Subscribe now!

Ongoing reading
Image sensors