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Article | REF: P3312 V1

Protein analysis or proteomics

Authors: Alexia ORTIZ, Caroline TOKARSKI, Christian ROLANDO

Publication date: September 10, 2011

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4. Quantification

4.1 Differential quantification

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4.1.1 Isotopic metabolic labeling

The metabolic isotope labelling technique is based on the use of modified culture media incorporating labelled amino acids in place of native amino acids. As a result, proteins derived from cells grown on these modified culture media incorporate the tagged amino acids in their sequences. As a result, this technique limits its field of application to proteins from cultured cells. Isotope incorporation methodology was first presented in 2002 by Matthias...

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