6. Glossary
microscopie plein champ; widefield microscopy
Instrumental method for rapid observation of thin samples with a wide field of view and limited resolution, using binoculars or a camera. The image is visualized by phase contrast, interference contrast, polarization or fluorescence labeling.
confocal microscopy; confocal microscopy
Instrumental method for observing fluorescently labeled samples. Linear fluorescence is generated by a precise wavelength from a lamp or laser. Selection of the fluorescence from the focal plane by means of an iris gives the system a "confocal" character, enhancing resolution performance.
multiphoton microscopy; multiphoton microscopy
Instrumental method for observing samples that may be labeled or show a break in structural symmetry. Non-linear...
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Bibliography
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Website
Linear absorption spectra of the most common fluorophores can be found by following one of the links below:
Thermo Fisher Scientific : http://www.thermofisher.com › home › labeling-chemistry
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