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2. Sample preparation
Thin slide preparation is a key aspect of transmission electron microscopy. As a rule, it requires a fair amount of patience and know-how. In the region of interest, the sample should be around 200 nm thick for conventional microscopy, or even 5 to 40 nm for atomic resolution techniques. In most cases, final thinning is performed on small 3 mm (sometimes 2.3 mm) diameter pellets, taken from 0.10 to 0.20 mm thick slides.
The first step is to cut the material into a blade. If the sample is large enough, slices 0.2 to 0.4 mm thick can be cut directly, using a diamond disk saw or electroerosion
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The Ultimate Scientific and Technical Reference