Article | REF: NM4050 V1

Mesoporous Silica Nanoparticles (MSN) and biological applications

Authors: Jean-Olivier DURAND, Laurence RAEHM

Publication date: July 10, 2009

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


Français

3. MSN for gene transfection

Gene transfection involves introducing an exogenous DNA fragment into the host cell. The MSNs are used to transport the DNA fragment into the cell, where it is bound to the MSNs by weak (electrostatic) bonds. Lin's group [28] encapsulated a marker, red-texas, in the nanoparticle mesopores and then plugged the pores by covalently binding a dendrimer: a generation 2 polyamidoamine. This dendrimer, bound to the nanoparticles, is positively charged and has the ability to bind negatively charged DNA plasmids. The plasmid pEGFP-C1, which expresses GFP, was thus bound to MSNs and the binding monitored by electrophoresis. MSNs are effective in protecting the plasmid from endonucleases. Transfection of HeLa cells with MSNs was performed and compared with commercial agents. Transfection is much more efficient with MSNs: the level...

You do not have access to this resource.

Exclusive to subscribers. 97% yet to be discovered!

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!


The Ultimate Scientific and Technical Reference

A Comprehensive Knowledge Base, with over 1,200 authors and 100 scientific advisors
+ More than 10,000 articles and 1,000 how-to sheets, over 800 new or updated articles every year
From design to prototyping, right through to industrialization, the reference for securing the development of your industrial projects

This article is included in

Nanosciences and nanotechnologies

This offer includes:

Knowledge Base

Updated and enriched with articles validated by our scientific committees

Services

A set of exclusive tools to complement the resources

Practical Path

Operational and didactic, to guarantee the acquisition of transversal skills

Doc & Quiz

Interactive articles with quizzes, for constructive reading

Subscribe now!

Ongoing reading
MSN for gene transfection