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3. Silk nanofibers
It is now easy to obtain and purify silk protein inexpensively from Bombyx mori cultures. As a result, fibroin from these silkworms is more widely used in biomedical applications than natural spider silk and biotechnologically-derived proteins.
3.1 Extraction and purification of fibroin
There are several methods for extracting fibroin, nevertheless, the most commonly used technique is dialysis. Bombyx mori cocoons are boiled for 30 minutes in a saline solution of sodium carbonate (Na 2 CO 3 ) at 0.02 mol/L. The fibrous network obtained is subsequently rinsed three times in water to extract some of the sericin. However, in order to extract this immunogenic protein completely,...
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Silk nanofibers
Different forms of silk matrices used in biomedical applications
Bibliography
Patents
Patent application number: 20100196447
Patent application title : SILK BIOMATERIALS AND METHODS OF USE THEREOF
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