Article | REF: F3480 V1

Long-term preservation of viable and functional biological systems

Authors: Sébastien DUPONT, Laurent BENEY, Patrick Gervais

Publication date: March 10, 2013

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4. Preservation by temperature reduction

Since molecular mobility, flexibility, plasticity and reactivity are strongly temperature-dependent, temperature reduction is an effective way of slowing down cellular metabolism.

Cold preservation is achieved in three main ways:

  • low and positive temperature maintenance ;

  • cryopreservation with crystal formation;

  • cryopreservation with vitrification.

Both cryopreservation methods use cryogenic temperatures at which gases liquefy at atmospheric pressure.

4.1 Hypothermic preservation

Lowering temperatures preserves molecules, cells, tissues and...

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Preservation by temperature reduction