2. NMR of proteins in solution

2.1 Features

A general introduction to nuclear magnetic resonance (NMR) is given in the article Nuclear Magnetic Resonance Spectrometry [P 2 880] in the treatise Analysis and Characterization. It will therefore not be repeated here. Here, we outline the main features of NMR studies of proteins in solution. As we shall see later, protein NMR generally uses four types of isotope: ¹ H, ² H, ^...

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NMR of proteins in solution

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page Proteins and peptides

Structural design

Bibliography

References

(1) - CANTOR (C.R.), SCHIMMEL (P.R.) - Biophysical Chemistry Part II. - WH Freeman et Company (1980).
(2) - THORNTON (J.M.), ORENGO (C.A.), TODD (A.E.), PEARL (F.M.) - Protein folds, functions and evolution. - J. Mol....

Websites

Databases

Protein Data Bank (PDB) https://www.rcsb.org/

BioMaguResBank (BMRB) http://www.bmrb.wisc.edu

NMR, molecular modeling

NMR Information Server http://spincore.com/nmrinfo

...

Manufacturers and suppliers

Fermenters are available to automatically control the culture of organisms for protein overexpression. These devices control pH, temperature and partial pressures of oxygen and carbon dioxide.

Fermenters

B Braun Biotech (Sartorius S.A) http://www.bbraunbiotech.com

Marked media and isotopes

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Software

The computers used for structural calculations are mainly UNIX-based workstations. This is due to the fact that the vast majority of programs are developed in a university environment under UNIX or LINUX, with a few exceptions (particularly molecule visualization programs) which run under Windows or MacOS.