3. Columns and stationary phases

SPC uses the vast majority of filled columns used in LC and, very exceptionally, capillary columns used in GC, where the stationary phase is immobilized.

3.1 Filled columns

The same geometries and variety of stationary phases are used in SPC as in LC and UHPLC, including the vast majority of chiral stationary phases (with the exception of stationary phases operating solely with aqueous eluent or at risk of denaturation in organic media, such as protein-grafted phases). However, wherever possible, working conditions (P, T and mobile phase flow rate) should be chosen to minimize pressure drop, which can lead to a density gradient in the column and hence to thermodynamic spreading of the solute band

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Columns and stationary phases

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Equipment

Bibliography

(1) - KLESPER (E.), CORWIN (A.H.), TURNER (D.A.) - High pressure gas chromatography above critical temperatures. - J. Org. Chem., 27, p. 700 (1962).
(2) - SMITH (R.M.) - Supercritical chromatography. - Series RSC Chromatography Monographs,...

Main suppliers

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Events

The "International Conference on Packed Column SFC" or SFC series is held every year, alternating between the USA and Europe.

International Symposium on Chromatography (ISC) biennial conference series. ISC 2018 will take place in Cannes-Mandelieu from September 23 to 27, 2018 under the chairmanship of Didier Thiébaut (ESPCI, Paris), Valérie Pichon (ESPCI, PAris) and Jean-Luc Veuthey (University of Geneva)....