Français
7. Conclusion
3D phase imaging is a label-free alternative to fluorescence microscopy. Unlike DIC or phase-contrast microscopy, digital holography provides quantitative phase information, but suffers from limited 3D resolution. Diffractive tomography is based on holography, but provides higher-resolution 3D imaging, by merging the different projections obtained on the object by varying a parameter (angle of illumination, angle of rotation of the object, wavelength). This technique makes it possible to measure refractive index and absorption in 3D. The availability of commercial systems makes this technique an interesting complement to fluorescence imaging, for scientists unable to mark their samples or looking for an alternative or complementary imaging mode.
The Ultimate Scientific and Technical Reference
This article is included in
Analysis and Characterization
This offer includes:
Knowledge Base
Updated and enriched with articles validated by our scientific committees
Services
A set of exclusive tools to complement the resources
Practical Path
Operational and didactic, to guarantee the acquisition of transversal skills
Doc & Quiz
Interactive articles with quizzes, for constructive reading
Conclusion
Glossary
Bibliography
Patents
Method and apparatus for simultaneous amplitude and quantitative phase contrast imaging by numerical reconstruction of digital holograms WO2000020929A1
Digital holographic microscope with fluid systems WO2014044823A1
Digital holographic microscope with electro-fluidic system, said electro-fluidic system and methods of use EP3196631A1
Apparatus and method...
Directory
Lyncée Tec – Digital Holographic Microscopes https://www.lynceetec.com/
Phasics, the phase control company http://phasicscorp.com/
Qmod Holographic And Fluorescence Microscopy. In One Single System
The Ultimate Scientific and Technical Reference
