Français
2. Different techniques to establish structures
Several biochemical, chemical and biophysical techniques are available to determine the different structural levels of an enzyme after purification. If these are well established, robotic and computerized tools can save considerable time and increase precision. The most common techniques to establish these structures are shown below.
2.1 Primary sequences: total, limited proteolysis, Edman sequencing and RT-PCR
To establish the primary sequence of a purified enzyme, the sample must be treated so that all covalent bonds (essentially disulfide bridges) involved in the tertiary structure are broken.
Once the enzymatic protein has been “linearized”, its overall amino acid composition can be determined. The protein solution is usually...
The Ultimate Scientific and Technical Reference
This article is included in
Drugs and pharmaceuticals
This offer includes:
Knowledge Base
Updated and enriched with articles validated by our scientific committees
Services
A set of exclusive tools to complement the resources
Practical Path
Operational and didactic, to guarantee the acquisition of transversal skills
Doc & Quiz
Interactive articles with quizzes, for constructive reading
Different techniques to establish structures
Structure and function of three enzymes
Bibliographical sources
Sources bibliographiques
The Ultimate Scientific and Technical Reference
