Article | REF: PHA1504 V1

Molecular enzymology - Enzymatic catalysis

Authors: Julien DUMOND, Serge KIRKIACHARIAN

Publication date: June 10, 2022

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2. Different mechanisms of enzymatic action

2.1 Enzymatic reaction to a substrate

The simplest enzymatic reaction is catalyzed by a single enzyme that converts a substrate into a product. The reaction is dependent on the concentration of substrate and product over time, and on the equilibrium to be reached.

The very nature of the enzyme (with coenzyme or release of several products) can generate irreversible reactions under given conditions.

In the case of a simple mechanism, the work of Michaelis and Menten, followed by Briggs and Haldane, has made it possible to establish the kinetics of the reaction transforming a substrate into a product

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Different mechanisms of enzymatic action