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3. Techniques for observing thick objects
3.1 Obstacles to direct observation
The study of biological tissues using fluorescence microscopy faces several obstacles. All cells exhibit autofluorescence in the absence of an applied probe. The main band of emission in the yellow-green band, between 500 and 600 nm, is due to flavoproteins when excitation is in the blue around 450 to 480 nm. NADH emits in the blue (460 to 470 nm) when excited in the UV around 360 nm.
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Techniques for observing thick objects
Advanced microscopy techniques
Bibliography
Software tools
Image capture and peripheral control
Metamorph http://www.moleculardevices.comcom/Products/Software/Meta-Imaging-Series/MetaMorph.html
Peripheral control
Websites
A wealth of information about microscopy can be found on the website published by the Federal University of Florida. http://www.micro.magnet.fsu.edu/
Historical and review articles on light microscopy from the journal Nature http://www.nature.com/milestones/light-microscopy
...Events
Thematic school on functional microscopy in biology http://www.mifobio.fr/
Directory
Manufacturers – Microscopes (non-exhaustive list)
Leica http://www.leica-microsystems.com/
Nikon http://www.nikoninstruments.com/
Olympus http://www.olympusmicro.com/
...
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